Welcome to the website of the Angiogenesis Laboratory Amsterdam 

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Angiogenesis is a key process in cancer. It is therefore a target in the development of novel anti-cancer therapies. The Angiogenesis Laboratory Amsterdam seeks to unravel the fundamental processes and mechanisms underlying angiogenesis and vascular development. The major aim is to use new knowledge and technology for the development of new treatment modalities in the clinic.

What's new in angiogenesis?

► VEGF-D promotes tumor metastasis - role of prostaglandins - Kamezis et al. Cancer Cell 2012

► Rapid decrease in chemotherapy delivery by anti-VEGF therapy - Van der Veldt et al. Cancer Cell 2012

► PDGF-B boosts tumor growth by EPO expression from stromal components - Xue et al. Nature Medicine 2012

► Micro-RNA regulation of angiogenesis and metastasis - Png et al. Nature

► Cancer stem cells reside in perivascular niche and get stimulated by VEGF through neuropilin-1 - Beck et al. Nature.

► Tumor hypoxia promotes recruitment of Tregs through CC-chemokine ligand 28 (CCL28) - Facciabene et al. Nature.

► Acetylation dependent fine tuning of endothelial Notch signaling - Guarani et al. Nature.

► Action mechanism of angiosuppression by Robo4 - Koch et al. Dev Cell.

► Macrophage polarization and vessel normalization regulated by HRG - Rolny et al. Cancer Cell

► Tumor blood vessels without endothelium. Ricci-Vitiani et al, Nature; Wang et al, Nature; El Hallani et al, Brain.

Special issue of Angiogenesis

Special issue of Angiogenesis 'Imaging of angiogenesis'

Guest editor: Dr. WJM. Mulder

Topics:

- Imaging vascular permeability and lymphatic drainage

- MR imaging to identify vulnerable atherosclerotic plaques

- Imaging angiogenesis in the brain after stroke

- Nanoparticle targeting in tumors

- Quantum dots for multimodal imaging of angiogenesis

- Optical imaging of angiogenesis

- MR imaging of head and neck squamous cell carcinoma

- Multimodal liposomes for imaging of angiogenesis

- Ultrasound based molecular imaging of angiogenesis

- Angiogenesis-specific perfluorocarbon nanoparticles

Methods, techniques and models available at the Angiogenesis Laboratory
Sorting of endothelial cells from (tumor) tissues Gene expression or proteomic profiling of tumor angiogenesis	Microvessel density and proliferating endothelial cell assessment Angiogenesis assessment in clinical samples
Endothelial cell migration Assessment of mobility and motility of endothelial cells	3-D endothelial cell tube formation The workhorse assay of the Angiogenesis Laboratory
2-D endothelial cell tube formation Rapid screening of compounds	In vivo angiogenesis in the chorioallantoic membrane of the chicken embryo Rapid in vivo screening
Molecular profiling of angiogenesis Species specific detection of >50 angiogenesis genes by real-time quantitative PCR.
Recent research
HMGB-1 a novel tumor endothelial marker Van Beijnum et al. Oncogene 2012	Blood platelets and tumor angiogenesis Sabrkhany et al. BBA Cancer, 2011
Tumor cell resistance against sunitinib. Gotink et al. Clin.Cancer Res. 2011	Angiostasis induced vascular normalization can improve photodynamic therapy. Nowak-Sliwinska et al. Cell Mol Life Sci, 2010
Photodynamic therapy Weiss et al. BBA Cancer, 2012	Neo-angiogenesis after photodynamic therapy. Nowak-Sliwinska et al. Angiogenesis, 2010.
Galectin-1 is an angiogenic growth factor. Thijssen et al., Cancer Research, 2010.	Synergistic targeting of alphavbeta3 integrin and galectin-1 with heteromultivalent paramagnetic liposomes for combined MR imaging and treatment of angiogenesis. Kluza et al. Nano Letters, 2010

Key papers of the Angiogenesis Laboratory

IL-21 a new angiogenesis inhibitor

Castermans et al., Blood, 2008

Isolation of tumor endothelial cells

Van Beijnum et al. Nature protocols, 2008

Quantum dot-based imaging of angiogenesis.

Mulder et al. Nano Letters, 2006.

Novel targets on tumor endothelial cells.

Van Beijnum et al. Blood, 2006.

Galectin-1 the receptor for anginex; a key molecule in angiogenesis

Thijssen et al, PNAS, 2006.

NF-kappaB in angiogenesis inhibition

Tabruyn & Griffioen, Cell Death Diff., 2007

News & events - The Angiogenesis Laboratory Amsterdam in the news (articles only in Dutch)

VUMC, November 2010

Medische Oncologie March, 2007 

De Volkskrant, September 9th, 2006

Last updated on

May 6th, 2012

Tracer, April 2009

Mare, Leids Universitair weekblad, March, 2008

Chemisch Tijdschrift, March, 2007 C2W Life Scieces,

Summum, December 20th, 2006